RE: Too much information

Posted by Lew (A) on November 7, 2020 at 07:20:09

In Reply to: RE: Too much information posted by dave slagle on November 7, 2020 at 05:50:30:

The second determinant of depth of field is magnification, as I am sure you know. You can see that any time you use a telephoto lens on a camera. The higher the magnification and the wider the lens opening, the less the depth of field. Even with a light microscope and the subject on a slide where it is essentially but not completely flat, one is constantly focusing up and down in order to see everything on the specimen from top surface to bottom surface. In electron microscopy the specimen is literally flat on a grid. All depth has been removed by the fixation process. That's with a conventional EM image. There are some modern and novel approaches that I don't really know much about that might allow for better visualization of depth.

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Topic - Cartridge retipping? - SgreenP@MSN.com 12:56:35 11/4/20 (25)

How many hours? nt - EdAInWestOC 05:45:07 11/6/20 (0)

RE: Cartridge retipping? - mondial 19:18:12 11/4/20 (2)

RE: Cartridge retipping? - Joe Backer 08:55:28 11/5/20 (0)

RE: Cartridge retipping? - Mick Wolfe 08:08:28 11/5/20 (0)

RE: Cartridge retipping? - Audioquest4life 17:23:49 11/4/20 (1)

RE: Cartridge retipping? - John Elison 19:02:31 11/4/20 (0)

RE: Cartridge retipping? - PAR 14:43:32 11/4/20 (17)

RE: Cartridge retipping? - pictureguy 15:56:56 11/4/20 (16)

RE: Cartridge retipping? - PAR 16:46:47 11/4/20 (15)

RE: Cartridge retipping? - pictureguy 19:41:32 11/4/20 (0)

And you have to sputter coat it in gold before the SEM - 13th Duke of Wymbourne 17:21:21 11/4/20 (13)

RE: And you have to sputter coat it in gold before the SEM - pictureguy 19:47:02 11/4/20 (12)

Too much information - Lew 08:16:11 11/5/20 (11)

RE: Too much information - pictureguy 08:55:17 11/5/20 (10)

RE: Too much information - Lew 09:23:06 11/5/20 (9)

That's why I now observe what a new stylus looks like under my - alaskahiatt 10:52:35 11/6/20 (0)

RE: Too much information - dave slagle 16:45:53 11/5/20 (6)

RE: Too much information - Lew 22:10:47 11/6/20 (2)

RE: Too much information - dave slagle 05:50:30 11/7/20 (1)

RE: Too much information - Lew 11/7/2007:20:09 11/7/20 (0)

RE: Too much information - John Elison 21:26:32 11/5/20 (2)

RE: Too much information - dave slagle 05:58:27 11/6/20 (1)

RE: Too much information - John Elison 16:08:15 11/6/20 (0)

RE: Too much information - pictureguy 12:31:56 11/5/20 (0)

RE: Cartridge retipping? - Dave Billinge 14:35:49 11/4/20 (0)

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RE: Too much information - Lew 11/7/2007:20:09 11/7/20 (0)

RE: Too much information

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